Fig. 2
7rh impedes outgrowth of xenografted Omm1 cells and MP41 cells patient-derived xenograft model in NOD-SCID mice. a–c Male NOD-SCID mice (4–6 weeks) bearing palpable Omm1 xenografted tumors were randomly administered with either vehicle (ddH2O:DMSO:EtOH:Cremophor EL = 90:2:4:4) or 7rh (25 mg/kg in vehicle, orally) every day for 14 days (n = 8 per group). a The tumor volumes estimated every other day measurement by caliper versus time were plotted. Data are shown as the mean ± SD (n = 8). b Representative tumors resected from mice treatment with vehicle or 7rh for 14 days are shown. c Weights of tumor from mice of each group are shown. Data are shown as the mean ± SD (n = 8). d The xenograft tissues from the vehicle- or 7rh-treated mice were subject to H&E staining and IHC analysis with anti-Ki67. Scale bar: 100 µm (H&E staining), 50 µm (IHC staining). e The total number of Ki67-positive cells (brown-stained nuclei, regardless of staining intensity were counted as positive) in three random microscopic fields was counted by Image-Pro Plus 6.0. Data are shown as the mean ± SD (n = 3). f Western blotting detection of DDR1, phospho-DDR1 and its downstream signal phospho-STAT3 in tumor tissues from each group of mice is shown. g–l The effect of 7rh on UM PDX model. Male NOD-SCID mice (4–6 weeks) bearing palpable MP41 cells xenografted tumors were randomly administered with either vehicle (ddH2O:DMSO:EtOH:Cremophor EL = 90:2:4:4) or 7rh (25 mg/kg in vehicle, orally) every day for 22 days. g The tumor volumes estimated every other day measurement by caliper versus time were plotted. Data are shown as the mean ± SD (n = 8). h, i The mice were sacrificed and tumors were collected, then tumors were photographed and weights were measured and analyzed. Data are shown as the mean ± SD (n = 8). j, k Tumor sections from two groups were subject to H&E staining and IHC analysis with anti-Ki67. The total number of Ki67-positive cells was counted. Data are shown as the mean ± SD (n = 3). Scale bar: 100 µm (H&E staining), 50 µm (IHC staining). l Western blotting analysis of cell lysates of tumors was performed to detect DDR1, phospho-DDR1 and its downstream signal phospho-STAT3 after vehicle or 7rh treatment. **P < 0.01; ***P < 0.001, Student’s t test for results in (a, c, e, g, i and k)
