Fig. 1
From: GPR160 is a potential biomarker associated with prostate cancer
Higher expression of GPR160 in cancerous human prostate tissues and relationship between GPR160 expression levels and clinical characteristics of enrolled patients. a Images of GPR160 expression detected by RNAscope® (mRNA, FISH)) and immunohistochemistry (protein, IHC). HE, hematoxylin and eosin, scale bars, 50 μm. b 143 samples containing both normal and cancerous tissues used for mRNA analysis with paired t test. c 167 samples containing cancerous and 143 samples containing normal tissues used for mRNA analysis with unpaired t test. d 112 samples containing both normal and cancerous tissues used for immunohistochemistry analysis with paired t test. e 140 samples containing cancerous and 112 samples containing normal tissues used analysis with unpaired t test. Data presented are means ± SEM of up to three independent reading; ***P < 0.0001. f Gleason scores vs. GPR160 mRNA levels in the prostate cancer tissue. g Disease stage vs. GPR160 mRNA levels in the prostate cancer tissue. h PSA levels in patients with elevated GPR160 mRNA transcription. i Age-related GPR160 protein intensity. j M scores in patients with or without GPR160 protein expression. Data shown are means ± SEM; *P < 0.05 using unpaired t test. k Expression of the epithelial–mesenchymal transition (EMT) hallmarks E-cadherin, N-cadherin, and vimentin as well as GPR160 in blank and GPR160 gene transfected cells. l Quantitative densitometric analysis of k. m Expression of the three EMT hallmarks and GPR160 in blank and GPR160 gene silenced cells. n Quantitative densitometric analysis of m. o Expression of the EMT hallmarks ZO-1, snail, and fibronectin 1 in blank and GPR160 gene silenced cells. p Quantitative densitometric analysis of o. q Expression of the EMT hallmarks ZO-1, snail, and fibronectin 1 in blank and GPR160 gene transfected RWPE-1 cells. r Quantitative densitometric analysis of q. s Expression of the EMT hallmarks ZO-1, snail, and fibronectin 1 in blank and GPR160 gene transfected WPMY-1 cells. t Quantitative densitometric analysis of s. u, Migration of GPR160 gene silenced or transfected cells. v Quantitative densitometric analysis of u. w Invasion of GPR160 gene silenced or transfected cells. x Quantitative densitometric analysis of w. y Wound-healing assay in GPR160 gene silenced or transfected cells. Data presented are means ± SEM obtained from three independent experiments; *P < 0.05, **P < 0.01, and ***P < 0.001 using Student’s t test
