Fig. 2
Activated memory B cells have a high proportion of antigen-labeled B cells. Gene expression distributions in distinct B cell states of established transcription factors (a), antigen-experienced (b), signaling receptors (c), immune activation related genes (d) and cell differentiation (e). f The group of antigen labeled quantity by quartile in B cell subgroups: <25% (Q1) showing as the low labeling quality; The 25–75% showing as the middle labeling quality; >75%(Q3) showing as the high labeling quality. g Proportion of antigen-labeled cells in memory B cells, Exprelow memory B cells, activated memory B cells, naive B cells and other B cells. Significance cut-offs: not significant (p > 0.05), *p < 0.05, **p < 0.001, ***p < 0.0001. h Binding of activated memory B cell antibodies to SARS-CoV-2 antigen by ELISA. PBS was used as a negative control. Data are represented as mean ± SD. Data are representative of two independent experiments. i–j Constituent ratio of antibody types derived from non-antigen labeled B cell subgroups (g) and antigen-labeled B cell subgroups (h). k Divergence from inferred germline gene sequences. The number of mutations of each clonotype relative to the inferred germline variable gene was counted. These numbers then were transformed into percent values and plotted as violin plots (COVID-19 VS COVID-19 antigen-labeled VS health)
