Fig. 5

Characteristics of SARS-CoV-2 reactive mAbs. SARS-CoV-2 antigen specificity as predicted was validated by ELISA for a subset of monoclonal antibodies to SARS-CoV-2. Data are represented as mean ± SD. Data are representative of two independent experiments. a SARS-CoV-2 RBD reactive mAbs, (b) SARS-CoV-2 NP reactive mAbs. c Maximum-likelihood phylogenetic tree of dominant clonotypes and antigen labeled antibodies’ heavy chains. Unrooted phylogenetic tree depicting the genetic relationships among all VH genes of antigen labeled antibodies. Branch lengths were scaled so that sequence relatedness can be readily assessed. Hvdj sequences with the same VH gene usage are shown in the same color at the clades. Hvdj sequences with the same antigen-labeled quantity are shown in the same color at the branch tips (red, blue and green means high, median and low antigen-labeled quantity respectively, and black means none). d Neutralization of C2767P3S and C14646P3S mAbs against pseudotyped SARS-CoV-2 virus in Huh-7 cells. Influenza relative mAbs CR9114 was used as a negative control. Data are represented as mean ± SEM. Data are representative of two independent experiments. e C2767P3S mAb was tested using the plaque reduction neutralization assay. DMEM was used as a negative control. Data are representative of two independent experiments. f Neutralization of C2767P3S mAb against live SARS-CoV-2 virus in Vero E6 cells. DMEM was used as a negative control. Data are represented as mean ± SEM. Data are representative of two independent experiments. g C14646P3S mAb was tested using the plaque reduction neutralization assay. DMEM was used as a negative control. Data are representative of two independent experiments. h Neutralization of C14646P3S mAb against live SARS-CoV-2 virus in Vero E6 cells. DMEM was used as a negative control. Data are represented as mean ± SEM. Data are representative of two independent experiments