Table 2 Examples of chemical spatiotemporal control of CRISPR gene editing

From: Spatiotemporal control of CRISPR/Cas9 gene editing

Control type

Cell or organism models

Edited gene

Key molecule / structure

Ref.

Small molecule activators

HEK-293T cell, mouse zygote, STF3A cell line, HEK293-GFP cell, N2A cell

GFP reporter gene, SOX2 gene, EMX1, PPP1R12C, VEGFA, ASCL

4-hydroxytamoxifen (4-HT), trimethoprim (TMP), rapamycin, azido-modified Cas9

27,63,66,67

Small molecule inhibitors

hPSC, HeLa cell, HEK-293T cell, K562 cell, E. coli, U2OS cell, NIH/3T3 cell

CD71 and CXCR4 gene, E. coli genome, endogenous IL1RN or NANOG gene, VEGFA, GFP or BFP reporter gene

AcrIIA1-4, ubiquitin ligase, unstable protein domains, DHFR and ER50

17,26,69,70,85

Bioresponsive delivery carrier

HEK-293 T cell, mouse hepatocytes, mouse liver, lung and spleen tissues

GFP reporter gene, mouse serum PCSK9 gene

bioreducible BAMEA-O16B lipid NP, lipid molecules with different charges

114,126

  1. HEK-293T cell human embryonic kidney 293T cell, STF3A a Cell that carries a Wnt-responsive luciferase reporter and also strongly expresses a Wnt ligand, N2A cell mouse neuroblastoma N2a cells, K562 cell human K562 erythroleukemia cell, NIH/3T3 mouse embryonic cells, hPSC human pluripotent stem cell, GFP green fluorescent protein, CD71 and CXCR4 cell surface transmembrane proteins gene, AcrIIA1-4 anti-CRISPR associated protein
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