Fig. 5: TFF3 promotes mucosal restitution via CD147-PTGS2-PGE2 signaling.

a Biophysical analysis of the mTFF3-mCD147^ECD interaction using SPR. b Western blotting analysis of the indicated proteins in mouse primary IECs treated with increasing amounts of mTFF3. SE, short exposure, 20 s; LE, long exposure, 1 min. c Quantification of PGE2 in the culture supernatant of mouse primary IECs treated with 0.28 μM of BSA or mTFF3. d Western blotting analyses of the indicated proteins in colonic tissue of the floxed mice (Cd147^fl/fl) or the intestinal epithelial-specific CD147 knockout mice (Cd147^ΔIEC). e Representative macroscopic views of colons from mice of the indicated genotypes treated with BSA or mTFF3 (7.7 μM protein/20 g weight) alone or in combination with etoricoxib (Etori, 0.2 mg/20 g weight). The graph shows the average length of the colon. The p-values in b–e were determined by using a two-tailed Student’s t-test. f Weight loss (expressed as a percentage of the initial weight) over time (n = 5). g Representative areas of healing ulceration. Black arrow, healing, and re-epithelialization are apparent; blue arrow, healing is not apparent