Fig. 2
From: AHR mediates the aflatoxin B1 toxicity associated with hepatocellular carcinoma
AHR deficiency increases the resistance of liver cancer cells to AFB1 treatment. a AHR sgRNA target sequence in the genome. b Western blot to validate AHR KD in PLC/PRF/5 and HuH7 cells. c Representative images of PLC/PRF/5 cells supplemented in medium containing different concentrations of AFB1 for 48 h. The images were acquired with a LEICA inverted microscope (DMI 4000B). d, e IC50 of AFB1 in AHR WT and AHR KD PLC/PRF/5 cells incubated with AFB1 for 48 h assessed with the CCK8 assay. AHR knockdown was achieved using CRISPR sgRNA technology in (d) and siRNA in (e). f Immunostaining for AFB1 adducts in AHR WT and AHR KD PLC/PRF/5 cells
