Fig. 3
From: AHR mediates the aflatoxin B1 toxicity associated with hepatocellular carcinoma
AFB1 increases the accumulation of long chain fatty acids. a The heatmap of regulated metabolites extracted from the AHR-WT-DMSO, AHR-KD-DMSO, AHR-WT-AFB1, and AHR-KD-AFB1 group in the negative ion mode. b Pathway enrichment analysis of regulated metabolites of the four groups in the negative ion mode. c, d The level of c PG(20:4_22:6)-H and d PG(18:1_22:5)-H was detected by LC–MS/MS. The results were expressed as mean ± SD. Data among multiple groups were analyzed using two-way ANOVA, *p < 0.05, **p < 0.01, and ***p < 0.001. e Representative fluorescence images of cells stained with Nile Red to detect LCFAs (600X). Nuclei are stained with DAPI (blue)
