Fig. 4 | Signal Transduction and Targeted Therapy

Fig. 4

From: A dual-role of SARS-CoV-2 nucleocapsid protein in regulating innate immune response

Fig. 4

Low-dose 2N protein inhibits IFN-I production through TRIM25. a TRIM25 knockout (sg25) and WT HepG2 cells in 24-well plate were transfected with an ISRE reporter and 0.25 μg 2N plasmids, together with or without poly(I:C). At 24 hpt, cells were harvested and luciferase activity was measured. b sg25 and HepG2 cells in 24-well plate were transfected with 0.25 μg 2N and poly(I:C). At 24 hpt, the mRNA levels of IFNA, IFNB1, ISG15, OAS1, and MxA were determined using qPCR. c sg25 and HepG2 cells were transfected with the plasmids as indicated. At 24 hpt, the luciferase activity were measured. d, e sg25 cells were co-transfected with the plasmids as indicated. At 24 hpt, the luciferase activity was measured. f sg25 and HepG2 cells were transfected with 0.25 μg Flag-2N or empty vector together with poly(I:C). At 24 hpt, the phosphorylation levels of IRF3, STAT, or STAT2 were detected by western blot. Results shown are the mean ± SD of at least three independent experiments

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