Fig. 8

SARS-CoV-2 N protein dually regulates viral replication. a, c Caco-2 cells were infected with SARS-CoV-2 at MOI of 0.01. At 6, 12, 24, and 48 hpi, total RNA was extracted. The mRNA expression of the viral N gene (a) and host IFNA, IFNB1, ISG15 (c) relative to GAPDH control were examined using qPCR. b Caco-2 cells were infected with SARS-CoV-2 as indicated as in a, HEK293T cells were transfected with the indicated 2N plasmids. At 24 hpt, and lysis of infected and transfected cells (50 μg/sample) were analyzed by immunoblot. d–f The HEK293T cells were transfected with 2N plasmids as indicated. The cells were infected with SeV at 24 hpt. After 20 h, cells were analyzed with immunofluorescence (Scale bar, 100 μm) (d), immunoblot (e), and flow cytometry (f). Results shown are the mean ± SD of at least three independent experiments