Fig. 4 | Signal Transduction and Targeted Therapy

Fig. 4

From: SARS-CoV-2 infection causes immunodeficiency in recovered patients by downregulating CD19 expression in B cells via enhancing B-cell metabolism

Fig. 4

SARS-CoV-2 infection alters the BCR signaling through affecting the B-cell early activation. Naive (CD27−) and memory (CD27+) B cells from COVID-19 recovered patients and healthy controls were activated with 10 µg/ml AF546–mB-Fab′–anti-Ig tethered to lipid bilayers at 37 °C for 3, 5 and 7 min. Then cells were fixed, permeabilized, and stained with Alexa Fluor 647 anti-CD27 and anti-pY (a, b), followed by Alexa Fluor 488 goat anti-mouse IgG. Cells were analyzed by Tirf microscope. Shown are representative images, the mean values of the B-cell contact area (c), and the MFI of pY (d) in the contact zone from three independent experiments. Scale bars, 2.5 μm. Statistical evaluation was performed using the two-tailed Student’s t-test. Statistical significance is indicated using asterisks: *p < 0.05,**p < 0.01, ***p < 0.001, and ****p < 0.0001

Back to article page