Fig. 1 | Signal Transduction and Targeted Therapy

Fig. 1

From: Mesenchymal stem cell-derived small extracellular vesicles mitigate oxidative stress-induced senescence in endothelial cells via regulation of miR-146a/Src

Fig. 1

Characterization of MSC-sEV and their active internalization by HUVECs. a Representative images of western blot analysis showing the biomarkers of sEV, including TSG101, CD63, and Alix. Calnexin was used as a negative control (MSC-sEV1, 2.1 mg protein/mL MSC-sEV; MSC-sEV2, 0.21 mg protein/mL MSC-sEV). b Transmission electron microscopic images of MSC-sEV (scale bar, 100 nm). c Nanoparticles tracking analysis reveals the particle distribution of exosomes in various sizes. Within every nanometer diameter sets, the value of the ordinate represents the mean particle number. d Schematic illustration of the different types of endocytosis and their respective inhibitor (Created with BioRender.com). e Uptake of MSC-sEV by HUVEC pretreated with signal inhibitors chlorpromazine (CPZ), nystatin, or 5-(N-ethyl-N-isopropyl) amiloride (EIPA) for 1 h. Cells were incubated with the indicated inhibitors for 1 h before and during the incubation with MSC-sEV. HUVECs incubated with 200 ng/μL PKH26-labeled MSC-sEV for the indicated times, and uptake of MSC-sEV was determined by fluorescence microscopy (scale bar, 100 μm)

Back to article page