Fig. 3
MSC-sEV rescued senescent HUVECs functions. HUVECs were incubated with (s + 200 ng/μL) or without (s + 0 ng/μL) MSC-sEV for 48 h after pretreated with H2O2 (50 μM, 2 h) compared with normal HUVEC (control). a Representative images of transwell migration assays of HUVECs (scale bar, 200 μm). b Quantitation of transwell migration assays of HUVECs. n = 3, **p < 0.01, ***p < 0.001. c Representative images wound-healing assay showing the migratory abilities of HUVECs and the image were taken at the indicated times (scale bar, 200 μm). d Quantitation of wound-healing assay at 12 h. n = 3, ***p < 0.001. e Representative images of in vitro angiogenesis assay (scale bar, 200 μm). f Quantitation of mean tube lengths and branching points in the HUVEC network. n = 3, **p < 0.01, ***p < 0.001. g Quantitation of IL-1 alpha, IL-6, and IL-8 released by HUVEC was detected through ELISA. n = 3, *p < 0.05, **p < 0.01, ***p < 0.001. h Mitochondrial respiratory capacity measured by O2 consumption rate (OCR) using a Seahorse analyzer. i Quantitation of basal OCR. n = 3, ***p < 0.001. j Quantitation of respiration capacity. n = 3, ***p < 0.001. k Quantitation of the ROS level of HUVEC measured by fluorometric intracellular ROS Kit on a flow cytometer. n = 3, **p < 0.01. l Quantitation of HUVEC proliferation evaluated by MTS kits with the OD value on Day 0, Day 1, Day 2, Day 3, and Day 4. n = 4, **p < 0.01, ***p < 0.001. m Representative images of hematoxylin staining and CD31 (red) and DAPI (blue) immunofluorescence staining in paraffin-embedded sections of Matrigel plugs. n Quantitation of tubes formed in vivo. Arrows indicate tubes formed in vivo. n = 3, **p < 0.01
