Fig. 5 | Signal Transduction and Targeted Therapy

Fig. 5

From: Mesenchymal stem cell-derived small extracellular vesicles mitigate oxidative stress-induced senescence in endothelial cells via regulation of miR-146a/Src

Fig. 5

MSC-sEV rescued senescent HUVEC through up-regulating miR-146a. a The top 20 miRNAs in MSC-sEV were examined by Human miRNA Microarray, and the counts per million reads of miRNA in sample 1 and 2. The expression of the whole miRNome was analyzed in triplicate in senescent HUVEC (Sample S) and MSC-sEV-treated senescent HUVEC (Sample E). b Heat map showed all miRNAs upregulated in MSC-sEV-treated senescent HUVEC compared with senescent HUVEC. c Heat map showed the miRNAs that the upregulated fold change≥2 in MSC-sEV-treated senescent HUVEC compared with senescent HUVEC. (S represents senescent HUVEC, E represents MSC-sEV treated senescent HUVEC). d Quantitative of four of the upregulated miRNAs in HUVEC by qRT-PCR. n = 3, *p < 0.05, ***p < 0.001. HUVECs were treated with H2O2 before transfection, added MSC-sEV after transfection, and observed the changes after 24 h. (S represents senescent HUVEC; NC represents senescent HUVECs transfected with NC control RNA before treated with MSC-sEV in (e–k). e Representative images of SA β-gal staining (scale bar, 200 μm). f Quantitation of SA β-gal-positive cells in HUVEC. g Western blots analysis showing the change of senescence markers P16, P21, P53, and LMNB1 in HUVEC. h Representative images of transwell migration assays of HUVECs (scale bar, 200 μm). i Quantitation of transwell migration assays of HUVECs. n = 3, **p < 0.01, ***p < 0.001. j Representative images of in vitro angiogenesis assay (scale bar, 200 μm). k Quantitation of mean tube lengths and branching points in the HUVEC network. n = 3, **p < 0.01, ***p < 0.001. Senescent HUVECs were transfected with the mimics of miR-146a or with NC control RNA and observed the changes after 24 h. l Representative images of SA β-gal staining (scale bar, 200 μm). m Quantitation of SA β-gal-positive cells in HUVEC. n = 3, ***p < 0.001. n Western blots analysis showing the change of senescence markers P16, P21, P53, and LMNB1 in HUVEC. o Representative images of transwell migration assays of HUVECs. (scale bar, 200 μm). p Quantitation of transwell migration assays of HUVECs. n = 3, *p < 0.05, ***p < 0.001. q Representative images of in vitro tube formation assay (scale bar, 200 μm). r Quantitation of mean tube lengths and branching points in the HUVEC network. n = 3, *p < 0.05, **p < 0.01, ***p < 0.001

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