Fig. 4
The in vivo expression of CB6 with VEEV-VRP-CB6 delivery. a Outline of the experimental design of the expression evaluation of VEEV-VRP-CB6 in BALB/c mice. b BALB/c mice received intranasal administration of 5 × 105 IU VEEV-VRP-CB6 were euthanized and the lungs were collected at 1, 3, 5, and 7 dpi. The lung tissue homogenates were used as the primary antibodies to test the Vero E6 cells expressing SARS-CoV-2 S protein with IFA assay, the purified CB6 antibody was used as positive control. Scale bars represent 50 μm. c The lung tissues collected at 3 days after the administration of VEEV-VRP-CB6 were subjected to IFA analysis to stain for the expression of CB6 mAb using Alexa FluorTM 568 conjugated goat anti-human IgG (H + L) and different cellular marker proteins (PDPN/SPC/CC10/β-IV-tubulin), respectively. Scale bars represent 10 μm. (n = 2 mice per group)
