Fig. 3: miR-200 family mediates the “RNA–RNA” crosstalk between HMGB1 and RICTOR. | Signal Transduction and Targeted Therapy

Fig. 3: miR-200 family mediates the “RNA–RNA” crosstalk between HMGB1 and RICTOR.

From: An RNA–RNA crosstalk network involving HMGB1 and RICTOR facilitates hepatocellular carcinoma tumorigenesis by promoting glutamine metabolism and impedes immunotherapy by PD-L1+ exosomes activity

Fig. 3

a Luciferase assays with reporter constructs containing the wild-type or mutant HMGB1/RICTOR 3′UTR downstream of the luciferase gene were performed after co-transfection with miR-200a/200b/429 mimics in HEK293T cells. *p < 0.05, **p < 0.01, ***p < 0.001. b The mRNA levels of HMGB1 and RICTOR were determined by qRT-PCR 48 h after transfection of 100 nM miR-200a/200b/429 mimics in indicated cells. **p < 0.01, ***p < 0.001. c RNA FISH assays detecting the cellular localization of HMGB1/RICTOR and miR-429 in indicated cells

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