Fig. 5

Suppression and stimulation of FSAP impairs or promotes BBB integrity and angiogenesis in cultures of mouse brain microvascular endothelial cells (mBMECs) after OGD, respectively. a–c The paracellular permeability and Trans Endothelial Electrical Resistance (TEER) value were measured to reflect the barrier property of the mBMECs monolayer after OGD. a Quantitative analysis of the intensity of infiltrated fluorescein sodium, b FITC-BSA, and c monolayer electrical resistance. Data is expressed as mean ± SD; n = 6 for each group; ***p < 0.001 versus control group, #p < 0.05, ##p < 0.01, ###p < 0.001 versus vehicle group by one-way ANOVA followed by Tukey’s multiple comparison tests. d–f Apoptotic cells were stained with annexin V-FITC and propidium iodide and analyzed using a FACS Calibur flow cytometer, and cell viability was measured by Cell Counting Kit-8. d Representative images of FACS analysis of apoptotic cells, e quantitative analysis of apoptotic rates, and f cell viability. Data is expressed as mean ± SD; n = 6 for each group; ***p < 0.001 versus control group, n.s. not significant, ##p < 0.01, ###p < 0.001 versus vehicle group by one-way ANOVA followed by Tukey’s multiple comparison tests. g Representative images of transwell migration assays, BrdU incorporation assays, and tube formation assays, and h quantitative analysis of migration cells, proliferative cells, branch length, branch counts, and junction counts. Data is expressed as mean ± SD; n = 6 for each group; ***p < 0.001 versus Control group, ###p < 0.001 versus Vehicle group by one-way ANOVA followed by Tukey’s multiple comparison tests