Fig. 6

Endothelial transcriptional changes following OGD and HMW-HA treatment. a, b The volcano plot for differentially genes (DEGs) expression. The x-axis represents the log2 (fold change), the y-axis represents the log10 (p value) of the repeated test results. The red region contains the upregulated genes, and the green region contains the downregulated ones. Three biological replicates were used for RNA sequencing analysis. c Venn diagrams of the numbers of upregulated and downregulated gene changes in the endothelial cells observed following OGD with or without HMW-HA treatment, depicting the overlap of changes found in different groups. d, e GO term enrichment analysis of DEGs between d control and vehicle group and between e vehicle and HWA-HA group. The six biological process GO terms related to angiogenesis process was shown. The y-axis represents GO biological terms. The X-axis represents Gene ratio. The size of the dot represents the number of genes under a specific term. The color of the dots represents the adjusted p-value. f The Heatmap image depicting transcriptional changes of the 38 selected genes in different groups. g Validation of transcriptional changes of the selected 38 genes using RT-qPCR analysis. Data is expressed as mean ± SD; n = 6 group; n.s. not significant, **p < 0.01, ***p < 0.001 versus control group, n.s. not significant, #p < 0.05, ##p < 0.01, ###p < 0.001 versus vehicle group by one-way ANOVA followed by Tukey’s multiple comparison tests