Fig. 6 | Signal Transduction and Targeted Therapy

Fig. 6

From: Natural killer cell-derived exosomal miR-1249-3p attenuates insulin resistance and inflammation in mouse models of type 2 diabetes

Fig. 6

MiR-1249-3p relieves insulin resistance and inflammation via the SKOR1-SMAD6-TLR4-NF-κB axis. a–c After transfection with a miR-1249-3p mimic, miR-NC, or specific siRNA to SKOR1 or SMAD6, qRT-PCR analysis of miR-1249-3p (a), SKOR1 (b) and SMAD6 (c) expression in 3T3-L1 adipocytes cells with the indicated treatments was performed. d, g The expression of p-p65 and p65 in 3T3-L1 adipocytes cells with the indicated treatments was performed by western blot. e, f, h IL-1β, IL-6, and TNF-α expression in 3T3-L1 adipocytes cells subjected to the indicated treatments was assessed by ELISA. i The signaling pathway through which NK-derived exosomal miR-1249-3p regulates insulin resistance in type 2 diabetes mice. Experiments were performed at least in triplicate, and the results are shown as the mean ± s.d. Student’s t-test was used to analyze the data. (*P < 0.05; **P < 0.01; ***P < 0.001)

Back to article page