Fig. 5

The metabolic regulation of myeloperoxidase, apelin, and myostatin in vitro. a–c AML12 cells were treated with myeloperoxidase (MPO), apelin, or myostatin for 24 h and subjected to real-time PCR (n = 6). d–f 3T3-L1 adipocytes were treated with myeloperoxidase (MPO), apelin, or myostatin for 24 h and subjected to real-time PCR (n = 3–4). g–i C2C12 myotubes were treated with MPO, apelin, or myostatin for 24 h and subjected to real-time PCR (n = 3–4). j–l HUVEC were treated with myeloperoxidase, apelin, or myostatin for 24 h and subjected to real-time PCR (n = 6). m–o AML12 cells were treated with MPO, apelin, or myostatin for 24 h following starvation with DMEM medium for 2 h, then 100 nM insulin was administrated for 15 min and subjected to western blot (n = 3, quantifications were shown below the blots). Error bars represent SEM. *P < 0.05; **P < 0.01; ***P < 0.001. See also Supplementary Fig. S5