Fig. 6

MPO, apelin, and myostatin were regulated by transcription factor REST. a Venn diagram of potential transcription factors that could bind to the promoter region of MPO, apelin, and myostatin from two online ChIP-seq datasets: TF mapper and Signaling Pathways. b–f HUVEC were infected with SARS-CoV-2 (MOI = 0.005) for 18 h and subjected to real-time PCR (n = 6). g–i HUVEC were transfected with FOXA1, MAFF, or REST plasmid for overexpression for 72 h and subjected to real-time PCR (n = 4). j–l HUVEC were infected with 10 nM control siRNA (si-NC), FOXA1 siRNA (si-FOXA1), MAFF siRNA (si-MAFF), REST siRNA (si-REST) for 72 h and subjected to real-time PCR (n = 4). m HUVEC were infected with 10 nM control siRNA (si-NC), REST siRNA (si-REST) for 48 h, then infected with SARS-CoV-2 (MOI = 0.005) for 24 h and subjected to real-time PCR (n = 4). Error bars represent SEM. *P < 0.05; **P < 0.01; ***P < 0.001. See also Supplementary Fig. S6