Fig. 10 | Signal Transduction and Targeted Therapy

Fig. 10

From: An overview of kinase downregulators and recent advances in discovery approaches

Fig. 10

Chemical proteomic strategies for kinase inhibitor’s selectivity analysis in native proteomes. In these technologies, the test compound is preincubated with cell lysate, and then probes are introduced to enrich the uninhibited kinases. For KiNativTM approach (left), biotinylated acyl phosphate probes that bind kinases covalently are utilized; in KinobeadTM (right), uninhibited kinases are precipitated with broad-spectrum kinases inhibitors immobilized on Kinobeads, and then enriched proteins or peptides are analyzed by LC-MS/MS

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