Fig. 6

Spatiotemporal heterogeneity and polyclonal dissemination in four patients with ancestral recurrence revealed by multi-region WGS. a Phylogenetic trees constructed from four patients with ancestral recurrence (total of 30 tumor samples). Trees were derived from somatic mutations (SNVs + indels) in genic region based on subclonal architectures. The numbers of all genome-wide somatic mutations per patient are labeled above the tree (the numbers of somatic mutations in genic region involved in constructing the phylogenetic trees are labeled in brackets). Lengths of lines are proportional to the number of mutations in each cluster. Quantification of tumor spatial and temporal heterogeneity of each patient is on the right of the corresponding phylogenetic tree. b Fishplot showing the tumor clonal evolution process from primary tumor to early-recurrent tumor. c Oval plots showing the subclonal structure of tumor samples from each patient. The diameter of each oval is proportional to the corresponding CCF value. Subclonal structures are illustrated by the nested ovals. d Clonal evolutionary history. The dashed box shows the predicted historical states during the tumor ancestral recurrence. Black arrows denote clonal evolution during primary tumor progression. Red arrows denote clonal evolution from the primary tumor to recurrent tumor. Arrows are labeled the acquisition of new subclones with orthogonal lines. Each colored line corresponds to a subclone