Fig. 4 | Signal Transduction and Targeted Therapy

Fig. 4

From: Infection of wild-type mice by SARS-CoV-2 B.1.351 variant indicates a possible novel cross-species transmission route

Fig. 4

Inflammatory responses in B.1.351-infected mice. a–c The hACE2 transgenic mice, BALB/c mice, and C57BL/6 mice were challenged with B.1.351 variant as in Fig. 2. The lungs of each mice in each time point were harvested and homogenized. Total RNAs from lungs were extracted and proceeded to RT-qPCR for quantifying the mRNA expression of each cytokine gene, which included Il1b, Il5, Il6, Il9, Il10, Il12a/Il12p70, Il17a, Ifng, Ccl4, Ccl5, Tnfa, Gcsf, Cxcl1, Gmcsf, Il1a, and Il3. The RT-qPCR results were normalized to mouse GAPDH. The relative expression of each gene was calculated as 2−ΔΔCt method (n = 4). d The cytokines in sera were also quantified by corresponding ELISA kits, including IL-6, IL-10, IL-1α, IL-1β, and CCL5 (n = 4). Data represented as mean ± SEM in quadruplicate. p Values were calculated by two-way ANOVA with Dunnett’s multiple comparisons test. ns = p ≥ 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

Back to article page