Fig. 4
From: Inactivated SARS-CoV-2 induces acute respiratory distress syndrome in human ACE2-transgenic mice
Lymphopenia is caused by inactivated SARS-CoV-2 in the lungs. a Immunofluorescence analysis of T cell accumulation in lung tissues on 3 dpi. Inactivated SARS-CoV-2 induced marked infiltration of T cells labeled as CD4- or CD8-positive with elevated CD103 expression. The treated hACE2 mice showed more CD8-positive T cell infiltration. Green, CD4; violet, CD8; red, CD103; blue, DAPI. b Flow cytometric analysis of lymphocytes in lung tissues on 3 dpi. The percentage of CD8-positive T cells were markedly increased in CD3-positive T cells. Inactivated SARS-CoV-2 induced activation of CD4-positive T cells, CD8-posituve T cells and NK cells (labeled as CD69+) with elevated expressions of IFN-γ and Granzyme B. Data represent the mean ± SEM. Significance is indicated by: ns, no significance; *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001
