Fig. 4 | Signal Transduction and Targeted Therapy

Fig. 4

From: Intracellular CYTL1, a novel tumor suppressor, stabilizes NDUFV1 to inhibit metabolic reprogramming in breast cancer

Fig. 4

Intracellular CYTL1 interacts with NDUFV1 and positively regulates its protein expression. a ATP and ADP levels and b NAD+ and NADH levels in the cell lysates from MDA-MB-231 cells stably expressing CYTL1 or ΔCYTL1 were determined using kit assays. c Co-IP analysis of the interaction between exogenous HA-CYTL1 or HA-ΔCYTL1 and exogenous Flag-NDUFV1 in transfected HEK293T cells. d Co-IP analysis of the interaction between endogenous NDUFV1 and exogenous HA-CYTL1 or HA-ΔCYTL1 in transfected HEK293T cells. IgG served as the negative control. Tubulin was used as a loading control. e Colocalization of HA-CYTL1 and endogenous NDUFV1 was detected by Immunofluorescence in transfected HEK293T cells. Scale bar, 5 μm. f, g NDUFV1 protein expression was determined by western blot in MDA-MB-231 cells transfected with f HA-CYTL1 or HA-ΔCYTL1 expressing plasmids or g the indicated shRNAs. Tubulin was used as a loading control. h Lactate production levels in CYTL1 or ΔCYTL1 overexpressing MDA-MB-231 cells cotransfected with the indicated shRNAs. i Glucose-uptake activity and j the OCR in MDA-MB-231 cells overexpressing NDUFV1. The data are shown as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001

Back to article page