Fig. 3

Lipid metabolism is aberrant in NPC cells with excessive PROCR activation. a GSEA analysis of the RNA-seq results comparing PROCR overexpressing cells and their counterpart control cells. The cells were harvested for RNA-seq after APC treatment. b mRNA expression of the lipid metabolism related genes with top ranked in the RNA-seq data by RT-qPCR. c Nile red staining of the cells with PROCR overexpression or knockout. The right panel shows the corresponding statistical analysis of the fluorescence intensity. d The cell content of triglycerides and cholesterol in PROCR overexpression or knockout cells. All of the cells were harvested after 48 h of APC treatment. e Left panel showed the PCA analysis of vector and PROCR GOF cell groups. Each group contains four samples. All of the samples were pretreated with APC. Right panel showed the differential lipid molecules analysis. Each column in the figure represents a difference ion, and each row represents a sample. Different colors indicate different intensities. f Western blotting analysis of lipid-related genes or stem cell markers expression in PROCR overexpression or knockout cells. ns, not significant, *P < 0.05, **P < 0.01