Fig. 2 | Signal Transduction and Targeted Therapy

Fig. 2

From: Neuronal NR4A1 deficiency drives complement-coordinated synaptic stripping by microglia in a mouse model of lupus

Fig. 2

Transcriptional profile revealing increased phagocytosis and complement signal activation in the MRL/lpr mouse brain. a Workflow of RNA-seq data analysis of the hippocampus in MRL/lpr and MRL/mpj mice, with a heat map of differentially expressed genes. The bar chart displays 1908 variable genes, which are some of the corresponding KEGG terms and have higher expression in the MRL/mpj- or MRL/lpr- hippocampus (bottom). KEGG, Kyoto Encyclopedia of Genes and Genomes. b and c Heat maps showing the relative expression of significantly altered genes (see “Methods”) generated from the hippocampal RNA-seq of MRL/mpj vs. MRL/lpr mice at 6 weeks of age. Each column represents an individual mouse. d and e Validation of select genes and pathways in a unique set of mice by qPCR. n = 5–6 mice per group. f and g Immunoblotting validation of C3 and C1q in the cortex and hippocampus of 6-week-old MRL/mpj (n = 4) and MRL/lpr (n = 5) mice.The data are the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; and ns, not significant according to one-way ANOVA with Tukey’s post hoc test in (d), (e), (g). See also Supplementary Figs. 3 and 4

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