Fig. 5
From: Preventing autosomal-dominant hearing loss in Bth mice with CRISPR/CasRx-based RNA editing
Injection of AAV-CasRx + sgRNA3 (~5 × 109 vg of AAV) protects hair cells and hair bundles. a Representative confocal images of 100 μm cochlear sections harvested 10 weeks after injection. Samples were stained with myosin7a (Red). The images are from Tmc1+/+, Tmc1Bth/+ + AAV-CasRx + sgRNA3, and Tmc1Bth/+ non-injected mice (n = 5 mice) at locations corresponding to 8 and 16 kHz. The IHCs and OHCs are indicated. Scale bar: 20 μm. b The number of OHCs (upper) and IHCs (lower) per 100 μm of the cochleae. Data are shown as the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. Statistical analysis was performed by two-way Sidak’s multiple comparisons test. c SEM images of the apical cochlear sensory epithelium showing the morphology of the hair cell bundles. Tmc1+/+, Tmc1Bth/+ + AAV-CasRx + sgRNA3, and Tmc1Bth/+ non-injected samples were collected 10 weeks after injection. Scale bars: 20 μm (upper); 3 μm (lower)
