Fig. 1

Screening the transcript factors (TFs) that regulate PAS1. a Flow chart depicts the screening process. siRNAs of 183 TFs were transfected into breast cancer cell MCF7. After 72 h, total RNA was extracted, followed by qRT-PCR to detect the level of PAS1. b The TFs were filtered out from the term of negative regulation of transcription from RNA polymerase II promoter, and the changes of PAS1 levels were shown. c Analysis of expression of five TFs in breast cancer patients’ samples obtained from Oncomine datasets. d Control and siRNAs were transfected into MDA-MB-231 and MCF7 cells. After 48 h, total RNA was extracted, followed qRT-PCR analysis