Fig. 5

PAS1 inhibits cancer cell growth and metastasis. a Control or PAS1-expressing lentiviruses were orthotopically injected into multiple mammary glands of PyMT mice. Tumor-growth curves were derived by measuring tumor volume once every 3 days Data are mean of tumor volume (control group, n = 45 tumors; PAS1 group, n = 46 tumors). b Lung-metastatic nodules were counted (n = 8/group). Data are means ± SEM by two-tailed t test. c Representative lung-metastasis specimens were sectioned and stained with H&E. d–g PH20 shRNAs were transfected into stable MCF-7-control/PAS1-shRNA cells. Wound healing scratch and migration assays were performed in the stable cells. Data are from three experiments by two-tailed t test. h The efficacy of PH20 shRNAs was detected by Western blot