Fig. 6

Combination treatment of PAS1-30nt-RNA and decitabine inhibits tumor growth and metastasis. a, b Cells were treated with different doses of decitabine for 48 h, followed by RT-qPCR and Western blot. Data represent the means ± SEM by one-way ANOVA analysis. c MDA-MB-231-Luc-D3H2LN cells (2 × 10⁶ cells) were inoculated onto the mammary fat pad of mice. After 2 weeks, the mice were divided into four groups (n = 4/group), and followed the injection of PAS1-30nt-RNA or/and decitabine. Representative in vivo bioluminescence images. d Bioluminescence-based quantitation of primary tumor sizes. Data are means ± SEM by two-tailed t test. e MDA-MB-231-Luc-D3H2LN cells (1.5 × 10⁶ cells) were injected into the tail vein of mice. After 1 weeks, the mice were divided into four groups (n = 3/group), and followed the injection of PAS1-30nt-RNA or/and decitabine. Representative in vivo bioluminescence images. f Bioluminescence-based quantitation of metastatic signals. Data are means ± SEM by two-tailed t test. g A working model