Fig. 6

Transcriptional dynamic analysis of acquired resistance to cetuximab. a Clustering genes by similar expression profiles in PDX_362 for time-series samples. b, d Gene set enrichment analysis (GSEA) was performed for transcript abundance for post-treatment versus pre-treatment: the differentially expressed genes displayed enrichment for the regulation of RAS by GAPs gene sets and regulation of the TP53 activity gene sets. c Gene ontology (GO) analysis of differentially expressed genes in post-treatment versus pre-treatment samples. e Sankey diagram illustrating changes of the expression components in the RAS signaling pathway through cetuximab treatment