Fig. 1

Arachidonic acid on the side chain of phospholipid is released after ischemia/reperfusion. a t-Distributed Stochastic Neighbor Embedding (tSNE) analysis shows remarkable stability of the instrument and good reproducibility of samples. b Heatmap analysis of the different expression (DE) lipids in contralateral (Contra) group and ipsilateral (Ipsi) group. The DE lipids were selected by VIP > 1, which was calculated by OPLS-DA analysis, and p value <0.05 (t test). c Different expression lipids in the Ipsi group. Values are shown as log2 fold change relative to the Contra group. Each dot denotes one lipid type, and the dot color represents lipid class, and the dot size shows significance (t test). d–g The abundance of PC (36:4, 16:0/20:4), PE (42:8, 22:4/20:4), PE (38:6, 18:2/20:4), and PE (40:7, 18:1/22:6) are significantly downregulated in Ipsi group. Data are mean ± SEM, n = 6. t test was performed. h The correlation among the 69 significantly changed lipids between Contra and Ipsi. The color of a node represents the ratio (Ipsi/Contra) of the lipids. The wathet edge shows a negative, and the orange edge indicates a positive correlation. i The ion characteristic fragments for the four reduced lipids. j The statistics of the fatty acid side chain of the four reduced lipids. k AA contents of the contralateral and ipsilateral hippocampus of mice were assayed 6 h after MCAO/R. Data are means ± SEM, n = 5. t test was performed