Fig. 2

Thrombin is upregulated after acute cerebral ischemia/reperfusion. a The unimodal distributions of the protein intensities suggests no obvious degradation in samples. b Distribution of log2-transformed intensity of identified proteins in 6 samples. Black presents Contra, and red denotes Ipsi. c Principal-component analysis shows a clear separation between Contra (red) and Ipsi (water blue). d Sample volcano plot for MCAO mice model showing –log10 (p value) and logFC values for all proteins with highlighting for those that are significantly upregulated (red dots) or downregulated (blue dots) after MCAO, and the most changed protein - prothrombin was labeled. Proteins in black are not significantly changed after MCAO. e Reactome enrichment for the 75 upregulated (ratio Ipsi/Contra >2, and p < 0.05, t test) and 111 downregulated (ratio Ipsi/Contra <0.5, and p < 0.05, t test) proteins, based on the Metascape.²³ f Protein-protein interaction (PPI) analysis for the 75 upregulated proteins. The size and color for the node represent the ratio of Ipsi/Contra. g Levels of prothrombin assayed by mass spectrometry in the contralateral and ipsilateral hippocampus of mice 6 h after MCAO/R. Data are means ± SEM, n = 3. t test was performed. h Thrombin protein levels were examined from the contralateral and ipsilateral hippocampus of mice 6 h after MCAO/R. Western blots were analyzed with Image J and normalized to β-actin expression. Data are means ± SEM, n = 5. t test was performed