Fig. 5

Platelet activation after ischemic stroke. a The statistic of Spearman coefficients for 59 samples in two technical workflow replicates. b The unimodal distributions of the protein intensity suggest no palpable degradation in the serum sample. c The distribution of peptides for quantified proteins. d The distribution of quantified protein numbers in samples. e tSNE analysis of the proteomics for 59 serum samples, and no batch effects were observed. healthy controls (HC), ischemic stroke (IS). f A volcano plot of the 362 proteins, quantified in > 50% samples, and imputed by random forest. g The significantly changed (p < 0.05 and FDR < 0.25) pathway in ischemic stroke patients, based on gene set enrichment analysis (GSEA). h, i GSEA analysis of common pathway of fibrin clot formation (h) and immunoregulatory interactions between a lymphoid and a non-lymphoid cell (i) gene sets enriched among 362 proteins. j A heatmap of GSEA enriched proteins in the common pathway of fibrin clot formation gene set from 362 proteins. k Protein–protein analysis of the 22 upregulated proteins in stroke. l Using mass spectrometry to detect plasma prothrombin abundance in clinical ischemic stroke (IS) patients and healthy controls (HC). Data are means ± SEM. HC, n = 27; IS, n = 32. t test was performed