Fig. 7

Modulating ACSL4 expression altered the outcomes of acute ischemic brain injury. a Schematic of the experimental paradigm. b The location of AAV injection and ACSL4 expression in the brain of mice were verified. c Representative images of TTC staining of EGFP and ACSL4 OE mice 24 h after 30 min of MCAO. Quantification of infarction volume indicated by TTC staining using Image J. Data are means ± SEM, n = 5 animals per group. t test was performed. d The neurological score was performed at 0 h, 6 h, 24 h, 3 days, and 5 days after MCAO/R. Data are means ± SEM. EGFP, n = 7; ACSL4 OE, n = 9. Two-way ANOVA with post-hoc Sidak test was performed. e The performance on the rotarod test was analyzed at 1 day, 3 days, and 5 days after MCAO surgery. Data are means ± SEM. Sham, n = 6; EGFP, n = 7; ACSL4 OE, n = 9. Two-way ANOVA with post-hoc Tukey test was performed. f Representative images of TTC staining of EGFP and ACSL4 KO mice 24 h after 60 min of MCAO. Quantification of infarction volume indicated by TTC staining using Image J. Data are means ± SEM, n = 5 animals per group. t test was performed. g Neurological scoring was performed at 0 h, 6 h, 24 h, 3 days, and 5 days after MCAO/R. Data are means ± SEM, n = 6 animals per group. Two-way ANOVA with post-hoc Sidak test was performed. h The performance on the rotarod test was analyzed at 1 day, 3 days, and 5 days after MCAO surgery. Data are means ± SEM, n = 6 animals per group. Two-way ANOVA with post-hoc Tukey test was performed. i Neurological scoring was performed at 1, 6, and 24 h after permanent-MCAO. Data are means ± SEM. EGFP, n = 5; ACSL4 KO, n = 6. Two-way ANOVA with post-hoc Sidak test was performed. j Representative images of TTC staining of EGFP and ACSL4 KO mice 24 h after permanent-MCAO. Quantification of infarction volume indicated by TTC staining using Image J. Data are means ± SEM. EGFP, n = 5; ACSL4 KO, n = 6. t test was performed. k Representative images obtained from PeriCam PSI System. The brighter area indicates higher blood perfusion. l Cortical blood flow changes before and after MCAO/R in rats. m The neurological score was performed at 0, 6, and 24 h after MCAO/R in rats. Data are means ± SEM. EGFP, n = 9; ACSL4 KO, n = 12. Two-way ANOVA with post-hoc Sidak test was performed. n Representative TTC-stained serial brain sections of rats 24 h after MCAO/R, where viable tissue stains red. Quantification of infarction volume indicated by TTC staining using Image J. Data are means ± SEM. EGFP, n = 9; ACSL4 KO, n = 12. t test was performed