Fig. 4

CNR2 facilitates tumor development by suppressing immune response. Tumor growth and survival were assessed in B16 (a), MC38 (b) and LLC (c) models in Cnr2^GFP and Cnr2^CKO mice. Data are representative of three independent experiments. Statistical significance was assessed by ordinary one-way ANOVA or log-rank (Mantel–Cox) test of survival curve, mean ± SEM. **P < 0.01. d Flow cytometric analysis of CD4⁺ and CD8⁺ T cells of Cnr2^GFP and Cnr2^CKO mice bearing B16 tumor. e The production of IFN‐γ in CD8⁺ T cells from B16 tumors was assessed by flow cytometric analysis. Data are representative of three independent experiments. Statistical significance was assessed by two-way ANOVA (d) or two-tailed unpaired Student’s t-test (e), mean ± SD, *P < 0.05, **P < 0.01. f Flow cytometry (left) and quantification (right) of PD-1, LAG3, and CD39 positive cells in Cnr2^GFP and Cnr2^CKO OT-I cells from B16-OVA tumors. Two-tailed unpaired Student’s t-test, mean ± SD, *P < 0.05, **P < 0.01.