Fig. 5
The tumor selectivity of OVM is modulated by a comprehensive effect of MXRA8 and ZAP. a Spearman’s correlation coefficient of cell viability under OVM treatment and ZAP expression levels. R indicates the Spearman’s correlation coefficient. mRNA expression levels of ZAP in tumor cell lines were retrieved from the CCLE database. b The protein expression levels of ZAP in HepG2, Hs578T, HeLa, and HT29 cells. c The infection rates of OVM-GFP in sgCtrl, sgCtrl + ZAP, ΔMXRA8 + ZAP Hs578T and HepG2 cells (MOI of 0.1) by flow cytometry (three experiments, n = 9; one-way ANOVA; mean ± s.d.). d, e The infection rates of HeLa cells transfected with siZAP or siNC and incubated with OVM-GFP virus (three experiments, n = 3; one-way ANOVA; mean ± s.d.). The protein expression levels of ZAP, NS3, and E1 were measured by Western blot. f The cell viability of HeLa tumor cells treated with OVM at the respective MOIs for 72 h was measured by MTT assay (three experiments, n = 9; one-way ANOVA; mean ± s.d.). *P < 0.05; **P < 0.01; ***P < 0.001
