Fig. 4 | Signal Transduction and Targeted Therapy

Fig. 4

From: Klotho in Osx+-mesenchymal progenitors exerts pro-osteogenic and anti-inflammatory effects during mandibular alveolar bone formation and repair

Fig. 4

Alveolar bone repair is attenuated in OsxCre;KLfl/fl mice. a, b μCT images and quantitative analyses of bone healing in the tooth extraction socket. White ellipses indicate extraction socket in maxilla. Blue arrowhead indicates the delayed healing of tooth socket. n = 3 in OsxCre group and n = 5 in OsxCre;KLfl/fl group. c–e H&E and TRAP staining of tooth extraction socket from control and OsxCre;KLfl/fl mice. Higher magnifications of boxed areas show less bone repair and more TRAP-positive cells in socket from OsxCre;KLfl/fl mice. n = 3 in OsxCre group and n = 6 in OsxCre;KLfl/fl group. f RUNX2 immunofluorescence staining and double fluorescence labeling of OSX:GFP and tdTomato show reduced Runx2 or OSX:GFP and tdTomato-positive osteoblasts in Klotho-deletion mice. g Quantification of RUNX2-positive cells or OSX:GFP and tdTomato double-positive cells in socket. n = 4 in OsxCre group and n = 7 in OsxCre;KLfl/fl group. *p < 0.05, **p < 0.01. All data are shown as the mean ± SEM. Scale bar, 500 μm (a), 200 μm (c), 100 μm (d), or 50 μm (f)

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