Fig. 4
Serum levels of ACE2, Ang II, and Ang-(1–7) in mice and expression of ADAM17 and ACE2 in the myocardium of mice and in differentiated H9c2 cells. a Representative Western blot images of ADAM17 and ACE2 protein expression in the myocardium of four groups of mice. b, c Quantitative analyses of ADAM17 and ACE2 expression in the myocardium of mice, n = 6. d Quantitative analysis of ADAM17 activity in the myocardium of mice, n = 6. e Quantitative analysis of ACE2 mRNA expression in the myocardium of mice, n = 6. f–h Quantitative analysis of serum levels of ACE2, Ang II, and Ang-(1–7) in mice, n = 6. Data were shown as mean ± SEM. *P < 0.05, **P < 0.01 vs. the A17fl/fl control group; #P < 0.05, ##P < 0.01 vs. the A17fl/fl DM group. i, j and l Representative Western blot images and quantitative analysis of ADAM17 and ACE2 protein expression in five groups of differentiated H9c2 cells treated with vehicle, mannitol, GP, GP + NC-siRNA and GP + ADAM17-siRNA, respectively. Mean values were derived from five independent experiments. k Quantitative analysis of ADAM17 activity in five groups of differentiated H9c2 cells treated as above. m, n Quantitative analysis of ACE2 mRNA level and ACE2 expression in the medium in five groups of differentiated H9c2 cells treated as above. Mean values were derived from five independent experiments. Data were shown as mean ± SEM. **P < 0.01, ***P < 0.001 vs. the vehicle group; ##P < 0.01, ###P < 0.001 vs. the GP + NC-siRNA group.
