Fig. 1
Upregulation of DDX39B correlates with aggressive phenotypes and poor prognosis in human CRC. A total of 37 up-regulated genes both in primary and metastatic CRC tumors were displayed as a heatmap (a) and by STRING network (b) analysis. N normal tissues; T: CRC tumors. c DDX39B transcripts in colon adenocarcinoma (COAD), rectal adenocarcinoma (READ) and normal tissues from the TCGA database were analyzed through an online tool (http://gepia2.cancer-pku.cn/). d, e DDX39B protein levels in a microarray of CRC and normal mucosal tissues were detected by immunohistochemistry (IHC) staining. d Representative images are shown, and e the relative staining intensities were analyzed by two-tailed unpaired t test. f DDX39B protein level in normal and colon cancer tissues from the Clinical Proteomic Tumor Analysis Consortium (CPTAC) database was analyzed through an online tool (http://ualcan.path.uab.edu/index.html). g, h DDX39B protein expression in primary CRCs and patient-paired liver metastases were detected by IHC. g Representative images are shown, and h the relative staining intensities were analyzed by two-tailed paired t test. i The correlation between DDX39B protein level and the overall survival of CRC patients was tested by Kaplan-Meier analysis. j Kaplan-Meier survival analysis for DDX39B transcripts in colon adenocarcinoma patients was carried out using an online tool (https://xenabrowser.net/). ***p < 0.001
