Fig. 1
Overview of aberrant alternative splicing events (AASEs) and related genes in LUAD. a Principal component analysis (PCA) based on gene expression profiles of LUAD and adjacent-normal samples in our integrated datasets. Each point represents a sample. b The pipeline of identifying high-confidence ASEs. c Ideographs of the seven types of ASEs. Each box indicates an exon. Exons connected by lower lines in each ideograph indicate exclusion transcripts, while upper lines represent inclusion transcripts. d The number of the seven types of AASEs and related genes identified in LUAD. e UpSet plot demonstrating the intersections among the seven types of AASEs in LUAD. f Summary of AASEs in LUAD samples in our integrated dataset. Bars show the proportion of the seven ASE types in each sample while points indicate the total number of AASEs identified in each sample. g Box plot displaying the detection frequency of the seven types of AASEs in LUAD samples. h Box plot showing that the PSI values of NUMB ES event were significantly higher in LUAD samples compared to adjacent-normal samples. i Western blot showing the expression of NUMB PRRL and NUMB PRRS in LUAD samples and paired adjacent-normal samples. j Kaplan–Meier survival curves comparing the overall survival of high and low NUMB PRRL/NUMB PRRS ratio subgroups. Patients were stratified into high (top 25th percentile) and low (bottom 25th percentile) subgroups based on their PSI value
