Fig. 2

Loss of IL-27 p28 enhances T cell responses after allo-HSCT. a, b BALB/c recipients were transplanted with 1 × 10⁷ WT BMs together with 5 × 10⁶ splenocytes from either C57BL/6 or CD11c-p28^f/f mice. Immune cell subsets were examined 14 days post-transplantation. Representative flow cytometric plots and quantification of activated T cells in spleens (among H2-Kb⁺H2-Kd^- cells) from recipients (n = 6 per group) are depicted. c–f Representative flow cytometric plots and quantification of IFN-γ-producing T cells (c, d) and TNF-α-producing T cells (e, f) in spleens (among H2-Kb⁺H2-Kd^- cells) from recipients (n = 6 per group) are depicted. g Serum from BALB/c recipients was collected 14 days post-transplantation and cytokines production was examined using LEGENDplex (n = 5–6 per group). h Splenocytes from aGVHD recipients were cocultured with irradiated BALB/c splenocytes. Proliferation rate was detected by ³H-TdR incorporation assay 3 days after coculture. i, j Treg cells were detected 14 days post-transplantation via FACS. Quantitative data (i) and representative plots (j) of donor Tregs are shown (n = 6 per group). k, l CFSE-labeled effector T cells (CD4⁺CD25⁻ T cells) were cocultured with Treg cells sorted from the spleens of C57BL/6 or CD11c-p28^f/f mice at the indicated ratios for 5 days. Representative figures (k) and frequency of cell proliferation are depicted (l) (n = 4 per group). m The frequency of IL-10⁺ Treg cells is shown (n = 4 per group). Data are representative of three independent experiments and presented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001