Fig. 1

Identifying HSPCs in ITP and HC bone marrows. a Schematic overview. Related to Figs. 1, 2, 4, 8, and 9. b UMAP plot showing cell contribution by samples. c Cell clusters were visualized using UMAP. Colors indicate cell types. Each dot represents one cell. HSC hematopoietic stem cells, MPP multipotent progenitors, GMP granulocyte and monocyte progenitors, NeuP neutrophil progenitors, MDP monocyte-dendritic-cell progenitors, EBMP eosinophil-basophil-mast-cell progenitors, CLP common lymphoid progenitors, pre-B pre-B cells, NK/Tp natural killer/T-cell progenitors, MEP megakaryocyte-erythroid progenitors, MkP megakaryocytic progenitors, EryP erythroid progenitors. Two unknown clusters could not be identified. Both were irrelevant to this study and were not analyzed later. d Stacked barplots show the percentage of sample contributions per annotated cell type (left) and the percentage of annotated cell type contributions per sample (right). e Heat map showing the scaled expression of top 10 marker genes in each cell cluster. Vital marker genes are highlighted on the right. f Violin plots showing the expression of specific marker genes in each cell cluster. Colors represent the cell clusters indicated in c. g Top 10 differentially expressed transcription factors (TFs) in each cell cluster. The vital TFs related to differentiation are listed on the right