Fig. 3

Transition state analysis of Mk/Ery lineages in ITP and HC samples. a Pseudotime-ordered analysis of HSC, MPP, MEP, MkP1, MkP2, and EryP populations from all ITP and HC samples. 2D graph of each cluster from HC (top) and ITP (bottom) samples are shown. b BEAM heat map depicting the expression of the branch-dependent genes over pseudotime. Genes are clustered to four modules based on expression patterns across pseudotime. The branch point shown in the middle of heat map is the beginning of pseudotime. Both sides of heat map are the ends of pseudotime. Color bar indicates the relative expression level. Directon1 matches the upper branch and Directon2 matches the lower branch as shown in Fig. 3a. c Representative GO: BP terms of each module. Adjusted p value < 0.05 was considered statistically significant for GO enrichment analysis. d Two-dimensional plots showing the dynamic expression of significantly enhanced genes in ITP along the pseudotime. e Two-dimensional plots showing the dynamic expression of scores for abnormality of complement system, complement activation, humoral immune response, humoral immune response mediated by circulating immunoglobulin, regulation of complement activation, and regulation of humoral immune response along with the pseudotime in ITP (red) and HC (blue) groups. The values of the y axis are the calculated GSVA scores. Pathways are selected from the GSEA enrichment results in ITP (NES > 1, NOM p val < 0.05, and FDR q val < 0.25)