Fig. 9

ITP-associated alterations in MkP subclusters. a TOP 5 GO: BP terms enriched in each MkP subcluster. b Heat maps showing the distribution of downregulated DEGs (left) and upregulated DEGs (right) between ITP and HC samples in each MkP subcluster. The gray bars on the left of the heat maps denote DEGs shared by at least two subclusters, while the others are subcluster-specific DEGs. c Volcano plots highlighting significant differences in gene expression between ITP and HC in MkP-IV (left) and MkP-V (right). Red points represent significantly upregulated genes, blue points represent significantly downregulated genes, and gray points represent non-differentially expressed genes. Genes with an adjusted p value < 0.05, log-transformed fold change absolute value >0.25, and minimum percentage >0.25 were considered as differentially expressed genes. d Boxplot showing the expression of DEGs in MkP-V. e Two-sided bar graph showing the top 10 enriched upregulated and downregulated GO terms in MkP-V in ITP. f GSEA plots showing representative pathways enriched in MkP-V from ITP versus HC. NES normalized enrichment score, NOM p value nominal p value, FDR q value false discovery rate q value. g GSEA plots showing representative pathways enriched in MkP-V from HC versus ITP. NES normalized enrichment score, NOM p value nominal p value, FDR q value false discovery rate q value