Fig. 3
From: Applications of human organoids in the personalized treatment for digestive diseases

Using gut organoids to study the impact of microbiota. Methods of infecting organoids with bacteria: (1) Microinjection. (2) Transwell/Plate-based 2D monolayer cultures of organoids. (3) Reversal of cell polarity of organoids. (4) Organoids-on-a-chip