Fig. 4

In vitro broad-spectrum safety and antiviral effect of lipase inhibitors. a–g Dose–response curves for half-maximal inhibitory concentration (IC₅₀) and half-maximal cytotoxicity concentration (CC₅₀) and selective index (SI) of atglistatin and CAY10499 against seven target RNA viruses. The viral yield in the cell supernatant was quantified by cell-culture immunofluorescence assay. Cytotoxicity of atglistatin and CAY10499 to each cell line was measured by MTT assay. h, i Effect of atglistatin and CAY10499 on reduction in viral genome copy numbers and viral infectivity titers for seven different RNA viruses in infected cells when treated just prior to the peak of lipase activity. Treatment times are shown in Supplementary Table 4. All data in the graphs are presented as arithmetic means ± SD from four independent experiments. One-way analysis of variance with Tukey’s correction for multiple comparisons