Fig. 4
Schematic diagram of the single-base substitution tool. a Fusion of Cas9n with adenosine deaminase or cytidine deaminase enables the introduction of point mutations in the genome, APOBEC1 induces a C to U mutation, and TadA induces an A to I mutation. b PE contains a 30 bp segment of pegRNA, including the PBS sequence and RT region. PBS binds to the DNA strand and synthesizes the complementary strand of the RT region in the presence of reverse transcriptase. PBS primer binding site, RT reverse transcriptase, pegRNA prime editing guide RNA. (Figure was created with Biorender.com)
